A Compact, High-Accuracy Cas9 with a Dinucleotide PAM for In Vivo Genome Editing (Molecular Cell)

Optimal viral delivery of Cas9 genome editors requires orthologs that are more compact than the commonly used SpyCas9, but existing platforms exhibit targeting restrictions, accuracy limitations, or both. Edraki et al. describe Nme2Cas9, which can edit a high density of genomic sites with little or no off-target mutagenesis.