Efficient Single-Strand Break Repair Requires Binding to Both Poly(ADP-Ribose) and DNA by the Central BRCT Domain of XRCC1 (Cell Reports)

Polo et al. map the binding sites for poly(ADP-ribose) and DNA of XRCC1 to non-overlapping surfaces of the central BRCT domain. Mutational disruption of the DNA binding site, which contains a common human polymorphism, affects recruitment and retention of XRCC1 at DNA damage and impairs single-strand break repair in vivo.