Unravelling Intratumoral Heterogeneity through High-Sensitivity Single-Cell Mutational Analysis and Parallel RNA Sequencing (Molecular Cell)

Rodriguez-Meira et al. developed TARGET-seq, a method for high-sensitivity mutational analysis and parallel RNA sequencing from the same single cell. Applied to 4,559 single cells, TARGET-seq unraveled transcriptional and genetic tumor heterogeneity in myeloproliferative neoplasm (MPN) stem and progenitor cells. TARGET-seq is a powerful tool for resolving the molecular signatures of genetically distinct tumor subclones.