The research was aimed at an overview and analysis of the demonstration activities in the Czech Republic dealing with the transfer of innovations for agricultural practice. Several methods were used to compile the national inventory, in particular the questionnaire survey method. The Czech part of the research was based on 30 questionnaires conducted among both farmers and agricultural organizations engaged in demonstration activities. The questions were focused on a specialization in agriculture, type of the management, connections with networks and their size, types of demonstration activities according to the types of farms and non-productive activities. The most common topics and purposes for demonstration in the Czech Republic are recorded for the category arable land with combined crops. Highly linked to sustainability are activities such as conservation agriculture on arable land with combined crops, root crops and fodders mainly due to a reduction of soil erosion within crop production and animal health management and welfare for dairy within livestock production. The Ministry of Agriculture has prepared a subsidy program to help farmers in the form of illustrative practical demonstrations of comprehensive sustainable farming practices. The main added values for the end-users, if the generated knowledge is implemented, are to strengthen the knowledge transfer system in agriculture, focusing on practical demonstrations and presentation of sustainable farming systems and soil protection in practice. However, the demonstration activities support mostly technological development and some individual ecological measures whereas the sustainability of agriculture as the complex process unifying the crop and animal production is not in the foreground.