Insights into kinesin-1 stepping dynamics from Brownian dynamics simulations and high-resolution tracking of gold nanoparticle-labeled motors (Biophysical Journal)

High-resolution tracking of gold nanoparticle-labeled proteins has emerged as a powerful technique for measuring the structural kinetics of processive enzymes and other biomacromolecules. These techniques use point-spread-function fitting methods borrowed from single-molecule fluorescence imaging to determine molecular positions below the diffraction limit. However, compared to fluorescence, gold nanoparticle tracking experiments are performed at significantly higher frame rates and utilize much larger probes.