Biodiesel has been one of the potential candidates in the field of renewable energy due to its biodegradability and non-toxicity in the natural environment. In addition, due to its high boiling point, the transportation of biodiesel is much safer than that of conventional fuel. However, the cost remains a challenge for the development of biodiesel. In this study, a homogenizer system, which can intensively minimize raw materials, is utilized as an effective approach to assist the production of both the calcium diglyceroxide (CaDG) catalyst and biodiesel. Several operational factors were evaluated to obtain the optimal conditions, and a desirable biodiesel conversion of 77.24% was achieved at a methanol-to-oil molar ratio of 7, with 2.0 g of calcium diglyceroxide, a rotation speed of 7000 rpm, a reaction temperature of 65 °C and a reaction period of 90 min.