Splice-Junction-Based Mapping of Alternative Isoforms in the Human Proteome (Cell Reports)

The translation and function of many alternative splicing events await confirmation at the protein level. Lau et al. use an integrated proteotranscriptomics approach to identify non-canonical and undocumented isoforms from 12 organs in the human proteome. Alternative isoforms interfere with functional sequence features and are differentially regulated during iPSC cardiomyocyte differentiation.