CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons (Molecular Cell)

Billon et al. describe a CRISPR-based approach to inactivate genes by directly converting four codons into STOP codons. The induction of STOP codons (iSTOP) can be rapidly monitored using restriction enzymes. iSTOP can be employed to inactivate eukaryotic genes on a genome-wide scale and model cancer-associated nonsense mutations.