Light Control of the Tet Gene Expression System in Mammalian Cells (Cell Reports)

Yamada et al. develop photoactivatable (PA)-Tet-OFF/ON systems for precise temporal and spatial control of cellular gene expression. The PA-Tet-OFF/ON can be applied to various cell types in vitro and in vivo, and their transcriptional activities are tightly regulated by blue light illumination and the small molecule Dox.