Comparative Analysis and Refinement of Human PSC-Derived Kidney Organoid Differentiation with Single-Cell Transcriptomics (Cell Stem Cell)

A strong characterization of cell types, lineages, and differentiation states present in human PSC-derived kidney organoids is critical to improve differentiation protocols. Wu et al. use single-cell transcriptomics to reveal non-renal cell types, describe lineage-specific expression of regulatory genes, and report a broadly applicable strategy to reduce off-target cell populations.