A Chemical Strategy for Protease Substrate Profiling (Cell Chemical Biology)

Proteases regulate countless (patho)physiological processes, but the identification of protease substrates is challenging. Here, Griswold et al. introduce a simple chemoproteomic strategy, termed CHOPS, for profiling protease substrates. Using CHOPS, the authors identify the cleavage specificities of proteases in cellular lysates and show that DPP9 preferentially processes short peptides.