Validation and Invalidation of Chemical Probes for the Human N-myristoyltransferases (Cell Chemical Biology)

Specific inhibitors of protein N-myristoylation are powerful probes for exploring the role of this post-translational modification in cells and have therapeutic potential. Kallemeijn et al. identify three flawed but commonly applied human N-myristoyltransferase inhibitors, and advocate two classes of high-affinity inhibitors as probes with a well-validated on-target cellular mode of action.