Mesoscale Modeling of Nucleosome-Binding Antibody PL2-6: Mono- vs. Bivalent Chromatin Complexes (Biophysical Journal)

Interactions of chromatin with bivalent immunoglobin (IgG) nucleosome-binding antibodies and their monovalent (papain-derived) Fab analogues are useful probes for examining chromatin conformational states. To help interpret antibody/chromatin interactions and explore how antibodies might compete for interactions with chromatin components, we incorporate coarse-grained PL2-6 antibody modeling into our mesoscale chromatin model. We analyze interactions and fiber structures for the antibody/chromatin complexes in open and condensed chromatin, with and without H1 linker histone (LH).