Kinetics and Fidelity of the Repair of Cas9-Induced Double-Strand DNA Breaks (Molecular Cell)

Brinkman et al. report a strategy to determine the rate and fidelity of double-strand break repair at single loci cut by Cas9. They also infer the contributions from different repair pathways. Cas9-induced breaks are repaired at variable but often slow rates, and the repair tends to be error prone.