Single-Unit Imaging of Membrane Protein-Embedded Nanodiscs from Two Oriented Sides by High-Speed Atomic Force Microscopy (Structure)

Haruyama et al. established a procedure for the observation of membrane proteins in nanodiscs by high-speed AFM in real time. Using the two-dimensional crystal of streptavidin as well as mica as a solid support surface, dynamic structural changes of membrane proteins in the lipid environment were visualized from two distinct angles.