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RSS FeedsIJMS, Vol. 21, Pages 593: SV40 Transfected Human Anterior Cruciate Ligament Derived Ligamentocytes--Suitable as a Human in Vitro Model for Ligament Reconstruction? (International Journal of Molecular Sciences)

 
 

16 january 2020 18:03:12

 
IJMS, Vol. 21, Pages 593: SV40 Transfected Human Anterior Cruciate Ligament Derived Ligamentocytes--Suitable as a Human in Vitro Model for Ligament Reconstruction? (International Journal of Molecular Sciences)
 


Cultured human primary cells have a limited lifespan undergoing dedifferentiation or senescence. Anterior cruciate ligaments (ACL) are hypocellular but tissue engineering (TE) requires high cell numbers. Simian virus (SV) 40 tumor (T) antigen expression could extend the lifespan of cells. This study aimed to identify cellular changes induced by SV40 expression in human ACL ligamentocytes by comparing them with non-transfected ligamentocytes and tissue of the same donor to assess their applicability as TE model. Human ACL ligamentocytes (40-year-old female donor after ACL rupture) were either transfected with a SV40 plasmid or remained non-transfected (control) before monitored for SV40 expression, survival, and DNA content. Protein expression of cultured ligamentocytes was compared with the donor tissue. Ligamentocyte spheroids were seeded on scaffolds embroidered either from polylactic acid (PLA) threads solely or combined PLA and poly (L-lactide-co-ε-caprolactone) (P(LA-CL)) threads. These scaffolds were further functionalized with fluorination and fibrillated collagen foam. Cell distribution and survival were monitored for up to five weeks. The transfected cells expressed the SV40 antigen throughout the entire observation time, but often exhibited random and incomplete cell divisions with significantly more dying cells, significantly more DNA and more numerous nucleoli than controls. The expression profile of non-transfected and SV40-positive ligamentocytes was similar. In contrast to controls, SV40-positive cells formed larger spheroids, produced less vimentin and focal adhesions and died on the scaffolds after 21 d. Functionalized scaffolds supported human ligamentocyte growth. SV40 antigen expressing ligamentocytes share many properties with their non-transfected counterparts suggesting them as a model, however, applicability for TE is limited.


 
43 viewsCategory: Biochemistry, Biophysics, Molecular Biology
 
IJMS, Vol. 21, Pages 595: Role of Cyclosporine in Gingival Hyperplasia: An In Vitro Study on Gingival Fibroblasts (International Journal of Molecular Sciences)
IJMS, Vol. 21, Pages 592: Inactivity and Skeletal Muscle Metabolism: A Vicious Cycle in Old Age (International Journal of Molecular Sciences)
 
 
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