Conditional Single Vector CRISPR/SaCas9 Viruses for Efficient Mutagenesis in the Adult Mouse Nervous System (Cell Reports)

Hunker et al. generate single adeno-associated viral vectors for conditional gene mutagenesis in the adult mouse nervous system with efficiencies equivalent to those of conventional gene inactivation strategies. On the basis of this efficacy, they provide a resource of Cre- and Flp-dependent constructs for targeting catecholamine, glutamate, and GABA neurotransmission.