Multiple Input Sensing and Signal Integration Using a Split Cas12a System (Molecular Cell)

Kempton et al. develop a split CRISPR-Cas12a toolbox for building multi-input, multi-output genetic circuits in mammalian cells. By splitting the Cas12a protein and effector into multiple components that can be individually controlled, they rationally engineer cells that can integrate information about multiple cues (e.g., tumor relevant) and execute programmed responses.