Tandem Paired Nicking Promotes Precise Genome Editing with Scarce Interference by p53 (Cell Reports)

Hyodo et al. describe a method of precise genome editing using Cas9 nickases, which barely introduces unwanted insertions and deletions at the edited genomic loci. This method neither activates nor is inhibited by p53 signalling, allowing safe genome editing and suggesting promise for this method in clinical genome engineering.