Physiological Tolerance to ssDNA Enables Strand Uncoupling during DNA Replication (Cell Reports)

Using specific POLA1 inhibitors, Ercilla et al. show that DNA synthesis works independently at leading and lagging strands and can be `uncoupled` in vivo. The authors show that cells can naturally tolerate ssDNA formation during DNA replication as long as it is protected by the surplus of RPA molecules.