Single-Domain Antibodies as Crystallization Chaperones to Enable Structure-Based Inhibitor Development for RBR E3 Ubiquitin Ligases (Cell Chemical Biology)

Tsai et al. identify single-domain antibodies (dAbs) that modulate the activity of the E3 ligase HOIP and facilitate crystallization of its catalytic RBR domain. HOIP/dAb crystals serve as a robust platform for ligand soaking and provide a novel tool for structure-based inhibitor development of E3 ligases.